hydrogen; palladium in tetrahydrofuran; ethyl acetate 0.5 h;

phosphoric acid; methyloxirane in pyridine 1.) 30 min, 2.) 40 deg C, 1 h;

silver orthophosphate; benzene anschl. mit wss. HCl und Methanol;

sucrose phosphorylase

recombinant galactokinase from Streptococcus pneumoniae TIGR4; magnesium chloride T=45°C; pH=8; 3 h; aq. bufferEnzymatic reaction; Kinetics; Time;

1: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction 2: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction View Scheme

1: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction 2: 3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide / water / 0.17 h / 45 °C / pH 7 / Enzymatic reaction View Scheme

1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; Cleland's reagent / aq. acetate buffer / 0.33 h / 30 °C / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 3: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme

1: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction 2: Ochromonas danica NIES-2142 1,3-β-D-glucan phosphorylase, native molecular mass estimated size-exclusion chromatography: ca. 250 kDa; potassium dihydrogenphosphate / aq. acetate buffer / 30 °C / pH 5.5 / |Enzymatic reaction View Scheme

3-(n-morpholino)-propane sulfonic acid; Paenibacillus sp. YM-1 laminaribiose phosphorylase; anhydrous phosphate; sodium hydroxide in water T=45°C; pH=7; 0.166667 h; Enzymatic reaction;