hydrogen; palladium on activated charcoal in methanol

trifluoroacetic acid T=20°C; 5 h;

1: 27 percent / Et3N / acetonitrile; methanol; tetrahydrofuran / 96 h / 20 °C 2: trifluoroacetic acid / 5 h / 20 °C View Scheme

1.1: Et3N / methanol; diethyl ether / 2 h / -10 °C 1.2: 95 percent / NH3 / methanol / 48 h 2.1: 27 percent / Et3N / acetonitrile; methanol; tetrahydrofuran / 96 h / 20 °C 3.1: trifluoroacetic acid / 5 h / 20 °C View Scheme

thiolsubtilisin in water; N,N-dimethyl-formamide Ambient temperaturepH 8.0 phosphate buffer containing PhB(OH)2 traces;