Prunus mume (R)-hydroxynitrile lyase; Na-citrate buffer in di-isopropyl ether T=25°C; pH=4.0; 21 h; Title compound not separated from byproducts;

1: 99 percent / DIBAL / diethyl ether / 1.) -70 deg C, 1 h, 2.) -70 -> -30 deg C, 1 h 2: 1.) oxalyl chloride, DMSO, 2.) Et3N / 1.) CH2Cl2, -60 deg C, 1 h, 2.) CH2Cl2, -20 deg C, 2 h 3: 13.7 g / R-oxynitrilase (E.C. 4.1.2.10) / various solvent(s) / 20 h View Scheme

1: 1.) oxalyl chloride, DMSO, 2.) Et3N / 1.) CH2Cl2, -60 deg C, 1 h, 2.) CH2Cl2, -20 deg C, 2 h 2: 13.7 g / R-oxynitrilase (E.C. 4.1.2.10) / various solvent(s) / 20 h View Scheme

R-oxynitrilase (E.C. 4.1.2.10) in various solvent(s) 20 h;