sulfuric acid in methanol

in methanol; hexane T=22°C; 1 h;

diazomethyl-trimethyl-silane in hexane; water T=22°C; 1 h;

1: nitrilase III; phosphate buffer / 22 h / 22 °C / pH 7 2: TMS-diazomethane / H2O; hexane / 1 h / 22 °C View Scheme

1: 3-O-(benzoyl)-glutaronitrile With Rhodococcus erythropolis NCIMB 11540 in phosphate buffer T=30°C; pH=7; 2: diazomethane in diethyl ether; ethanol Further stages. Title compound not separated from byproducts.;

hydrogen fluoride in acetonitrile 48 h; Ambient temperature;

1: 1 N aq. HCl / methanol / 2 h / Ambient temperature 2: imidazole / dimethylformamide / 48 h / Ambient temperature 3: n-BuLi / tetrahydrofuran / -78 deg C; room temp., overnight 4: 98 percent / NEt3, 4-DMAP / CH2Cl2 / 0.67 h / Ambient temperature 5: 94 percent / methanol / 0.33 h / Ambient temperature 6: 40percent HF / acetonitrile / 48 h / Ambient temperature View Scheme

1: 98 percent / NEt3, 4-DMAP / CH2Cl2 / 0.67 h / Ambient temperature 2: 94 percent / methanol / 0.33 h / Ambient temperature 3: 40percent HF / acetonitrile / 48 h / Ambient temperature View Scheme

1: 1-chloro-3-phenoxy-2-propanone With alcohol dehydrogenase from Lactobacillus brevis; NADPH; isopropyl alcohol T=30°C; pH=7.5; 6 h; aq. bufferEnzymatic reaction; 2: sodium cyanide With halohydrin dehalogenase from Mycobacterium sp. GP1 T=30°C; pH=7.5; 18 h; aq. bufferEnzymatic reaction; optical yield given as percent ee;

halohydrin dehalogenase Trp249Phe mutant; Tris-SO4 buffer in dimethyl sulfoxide T=22°C; pH=7.5; 1.5 h; Product distribution; Further Variations:Reagentsreaction times;