Tris-HCl buffer; carboxypeptidase G; zinc(II) chloride T=30°C; Hydrolysis; pH=3.7; 336000 h;

sodium hydroxide Hydrolysis;

1.1: 67 percent / tetramethylguanidine / dimethylsulfoxide / 9 h / 25 °C 2.1: tetrahydrofuran / 10.5 h / 0 - 25 °C 2.2: 123 g / ice / tetrahydrofuran / 3 h / 25 °C 3.1: 48 percent / aq. NaOH View Scheme

hydrogenchloride; carboxypeptidase G; Tris buffer; zinc(II) chloride 120 h; Ambient temperature;

sodium hydroxide Hydrolysis;

1: 89 percent / aq. cesium carbonate / dimethylformamide / 5 h / 25 °C 2: 32 percent / aq. NaOH View Scheme

sodium methylate; ethylene glycol

ethanol Loesung vom pH 4;

1: 62 percent / NaOH, H2O / 4 h / Ambient temperature 2: 2.) Me2NH*BH3 / 1.) glacial acetic acid, r.t., 15 min, 2.) glacial acetic acid, r.t., 20 min; 60 deg C, 10 min 3: 75 percent / trifluoroacetic acid / 1.5 h / Heating 4: NEt3 / dimethylformamide / 0.25 h / 5 °C 5: dimethylformamide / 72 h / Ambient temperature 6: 10 percent / NaOH, H2O / 1.5 h / Heating View Scheme

1: 1.) n-BuLi / 1.) ether, hexane, -78 deg C, 2 h, 2.) ether, hexane, -78 deg C to r.t. 2: 62 percent / NaOH, H2O / 4 h / Ambient temperature 3: 2.) Me2NH*BH3 / 1.) glacial acetic acid, r.t., 15 min, 2.) glacial acetic acid, r.t., 20 min; 60 deg C, 10 min 4: 75 percent / trifluoroacetic acid / 1.5 h / Heating 5: NEt3 / dimethylformamide / 0.25 h / 5 °C 6: dimethylformamide / 72 h / Ambient temperature 7: 10 percent / NaOH, H2O / 1.5 h / Heating View Scheme