反应条件
1: nicotinamide adenine dinucleotide; pyridoxal 5'-phosphate; [3H]-L-Alanine; ammonium chloride; L-alanine dehydrogenase; alcohol dehydrogenase from Bacillus stearothermophilus; ω-transaminase from Chromobacterium violaceum; sodium hydroxide / water; ethylene glycol dimethyl ether / 24 h / 20 °C / pH 8.5 / |Enzymatic reaction
2: triethylamine / water; acetonitrile / 1 h / 45 °C
View Scheme
参考文献
Sattler, Johann H.; Fuchs, Michael; Tauber, Katharina; Mutti, Francesco G.; Faber, Kurt; Pfeffer, Jan; Haas, Thomas; Kroutil, Wolfgang
Angewandte Chemie - International Edition, 2012 , vol. 51, # 36 p. 9156 - 9159
Angew. Chem., 2012 , vol. 124, # 36 p. 9290 - 9293