His-tagged Pseudomonas sp. metallo-β-lactamase-type alkylsulfatase Pisa1; water; tris(hydroxymethyl)methylamine hydrochloride T=30°C; pH=8.2; 6 h; Enzymatic reaction;

1: His-tagged Pseudomonas sp. metallo-β-lactamase-type alkylsulfatase Pisa1; water; tris(hydroxymethyl)methylamine hydrochloride / 6 h / 30 °C / pH 8.2 / Enzymatic reaction 2: 4-(N,N-dimethlyamino)pyridine / 18 h / 30 °C View Scheme

sulfur trioxide in 1,2-dichloro-ethane T=25°C; ΔH(formation of product); Thermodynamic data;