5-L-谷氨酰-L-半胱氨酸合成路线 （共 15 条）
反应条件
1: 1.) BSTFA / 1.) CH3CN, 50 min, 2.) CH3CN 2: 2.0 M NH2NH2*H2O, Et3N / methanol; H2O 3: 1.) Hg(OAc)2, 2.) H2S (gas) / 1.) H2O, 38-41 deg C, 2.5 h, 2.) H2O, RT, 45 min View Scheme
参考文献
Journal of Medicinal Chemistry, , vol. 39, # 8 p. 1676 - 1681
参考文献
Journal of the American Chemical Society, , vol. 80, p. 1158,1162
反应条件
pyruvate kinase; NADH-H+; Phosphoenolpyruvate; (Tris)-HCl buffer; adenosine 5'-triphosphoric acid; magnesium chloride; lactate dehydrogenase; γ-glutamylcysteine synthtase T=25°C; 1 h;
参考文献
Agricultural and Biological Chemistry, , vol. 52, # 10 p. 2633 - 2634
反应条件
recombinant (His)6-tagged Escherichia coli K12 W3110 γ-glutamylcysteine synthetase; magnesium sulfate; adenosine 5'-triphosphoric acid in water T=37°C; pH=8.5; 1 h; aq. bufferEnzymatic reaction;
参考文献
Bioscience, Biotechnology and Biochemistry, , vol. 73, # 12 p. 2677 - 2683
反应条件
formic acid; zinc T=35 - 40°C;
参考文献
Glutathione Symp. 1953 S.82
Biochemical Preparations, , vol. 9, p. 53
反应条件
hydrogen sulfide; mercury(II) diacetate 1.) H2O, 38-41 deg C, 2.5 h, 2.) H2O, RT, 45 min; Multistep reaction;
参考文献
Journal of Medicinal Chemistry, , vol. 39, # 8 p. 1676 - 1681
反应条件
sodium hydroxide; 2-(n-morpholino)ethanesulfonic acid; yeast carboxypeptidase Y EC 3.4.16.5; DL-threo-1,4-dimercapto-2,3-butanediol in water T=30°C; pH=5.5; 0.5 h; Enzyme kinetics;
参考文献
Bioscience, biotechnology, and biochemistry, , vol. 67, # 2 p. 434 - 437
反应条件
sodium hydroxide; 2-(n-morpholino)ethanesulfonic acid; yeast carboxypeptidase Y EC 3.4.16.5; DL-threo-1,4-dimercapto-2,3-butanediol in water T=30°C; pH=5.5; 0.5 h; Enzyme kinetics; Further Variations:pH-valuesReagents;
参考文献
Bioscience, biotechnology, and biochemistry, , vol. 67, # 2 p. 434 - 437
反应条件
1: carboxypeptidase-substance/s 2: zinc; aqueous formic acid / 35 - 40 °C View Scheme
参考文献
Biochemical Preparations, , vol. 9, p. 53
反应条件
ueber mehrere Stufen;
参考文献
Journal of Biological Chemistry, , vol. 199, p. 407
