thiamine diphosphate; magnesium sulfate in various solvent(s) T=30°C; Rearrangement; pH=7.5; 7 h; Enzymatic reactioninitial compound concentration 1, 10, 50 and 100 mM; reaction was carried out in Tris-HCl buffer;

transamination;

acetate buffer in water T=75°C; different buffers: pH 0.59 to 8.32; kH/kD; different temp.; Ea, ΔH(excit.), ΔS(excit.); MechanismKineticsThermodynamic data;

PEP phosphomutase in water T=25°C; Kinetics;