potassium carbonate in methanol 0.25 h;

β-glucosidase (EC No. 3.2.1.21) in water T=37°C; 2 h;

culture nutrient solution; Turnera angustifolia Miller shoots in water hydroxylation; 72 h; illumination;

1: 31 percent / BF3*Et2O / CH2Cl2 / 1.) -50 deg C, 15 min,2.) -50 deg C to room temperature, 3 h 2: 1.) COCl)2, DMSO, 2.) Et3N / 1.) CH2Cl2, -78 deg C, 10 min, 2.) -78 deg C, 15 min 3: 80 percent / K2CO3 / methanol / 0.25 h View Scheme

1: 1.) COCl)2, DMSO, 2.) Et3N / 1.) CH2Cl2, -78 deg C, 10 min, 2.) -78 deg C, 15 min 2: 80 percent / K2CO3 / methanol / 0.25 h View Scheme

hydrogenchloride 1 h; Ambient temperature; Product distribution;

water; β-glucosidase T=30 - 70°C; enzymatic hydrolysis: β-glucosidase from Manihot esculante Crantz cv. Merah Jambu; leaf enzyme: ΔEact=3.0 kcal/mol; peel enzyme: ΔEact=4.0 kcal/mol; tuber cortex enzyme ΔEact=3.5 kcal/mol; pH 3-7, 6-8; Thermodynamic data;