pyridoxal 5'-phosphate; tris(hydroxymethyl)methylamine hydrochloride; MSG; Aeromonas hydrophila IFO3820 in water; toluene T=30°C; pH=7.6; 16 h; Enzymatic reactionCulture medium; Product distribution / selectivity;

1: With sodium hydroxide in water T=35°C; Aldol Condensation; pH=11.0 - 11.1; 6.5 - 16 h; 2: With hydrogenchloride in water pH=6.91; Product distribution / selectivity;

sodium hydroxide; sodium carbonate in water T=20°C; pH=12.4; 2 h;

1: With potassium hydroxide in water T=35°C; 24 h; 2: With hydrogenchloride in water pH=7.0; Product distribution / selectivity;

1: With magnesium chloride; pyridoxal 5'-phosphate T=30°C; pH=7.5 - 7.8; Inert atmospherephosphate bufferEnzymatic reaction; 2: With [3H]-L-Alanine T=30°C; pH=7.5 - 7.8; Inert atmosphereEnzymatic reaction; Product distribution / selectivity;

1: With N-[2-hydroxyethyl]piperazine-N'-[3-propanesulfonic acid]; sodium hydroxide; magnesium chloride; pyridoxal 5'-phosphate T=25°C; pH=8.4; Inert atmospherephosphate bufferEnzymatic reaction; 2: With (R)-alanine T=25°C; Inert atmosphereEnzymatic reaction; Product distribution / selectivity;

(R)-alanine; sodium hydroxide; magnesium chloride; pyridoxal 5'-phosphate T=25°C; pH=7.8; 24 h; Inert atmospherephosphate bufferEnzymatic reactionTween 80; Product distribution / selectivity;

1: With sodium hydroxide; hydroxylamine hydrochloride in water T=5 - 20°C; pH=7; 4 - 17.5 h; 2: With hydrogenchloride in water pH=2; Product distribution / selectivity;