Diphosphate; recombinant Arabidopsis N-acetylglucosamine-1-phosphate uridylyltransferase-1; magnesium chloride T=37°C; pH=7.6; 0.166667 h; aq. bufferEnzymatic reaction; Kinetics; ConcentrationReagent/catalyst;

Diphosphate; recombinant Arabidopsis N-acetylglucosamine-1-phosphate uridylyltransferase-1; magnesium chloride T=37°C; pH=7.6; 0.166667 h; aq. bufferEnzymatic reaction; Kinetics; Reagent/catalystConcentration;

ManNAc kinase; UDP-N-acetylglucosamine 2-epimerase in phosphate buffer T=37°C; Enzyme kinetics;

deuterated phosphate buffer; ManNAc kinase; UDP-N-acetylglucosamine 2-epimerase in phosphate buffer T=25°C; Enzyme kinetics;

Multi-step reaction with 3 steps 1: N-acetylglucosamine kinase (GlcK, C. albicans)2: N-acetylglucosamine phosphate mutase (Agm1, S. cerevisiae)3: 92 percent / GlcNAc; PEP; ATP / Glc-1,6-dP1; MgCl2 / various solvents / 20 h