反应条件
tris-[2-carboxyethyl]-phosphine; His6-streptavidin-tobacco etch virus-human prolyl hydroxylase domain isoenzyme 2 (1-426); potassium chloride; oxygen; iron(II) chloride; ascorbic acid powder; 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid; BSA in water
T=25°C; pH=7.5; Enzymatic reaction; Kinetics;
参考文献
Pappalardi, Melissa B.; McNulty, Dean E.; Martin, John D; Fisher, Kelly E.; Jiang, Yong; Burns, Matthew C.; Zhao, Huizhen; Ho, Thau; Sweitzer, Sharon; Schwartz, Benjamin; Annan, Roland S.; Copeland, Robert A.; Tummino, Peter J.; Luo, Lusong
Biochemical Journal, 2011 , vol. 436, # 2 p. 363 - 369