ethylenediaminetetraacetic acid; toluene-4-sulfonic acid; threo-1,4-dimercapto-2,3-butanediol; papain in N,N-dimethyl-formamide T=25°C; pH=8; 0.25 h; aq. bufferEnzymatic reaction;

α-chymotripsin on Celite; Tris buffer; TEA in acetonitrile T=30°C; 48 h;

α-chymotrypsin immobilised on Celite; Tris buffer; triethylamine in acetonitrile T=30°C; pH=7.8; 48 h;

α-chymotripsin on Celite; Tris buffer; TEA in acetonitrile T=30°C; 48 h;