nucleoside deoxyribosyltransferase-II; anhydrous trisodium citrate T=40°C; pH=6.4; 42 h; Enzymatic reaction;

ammonium hydroxide T=55°C; 38 h;

1: 97 percent / pyridine / 6 h / Ambient temperature 2: DMAP / acetonitrile / 1 h / Ambient temperature 3: 1.) AIBN, Bu3SnH, 2.) TBAF / 1.) toluene, 80 deg C, overnight, 2.) toluene, THF, 80 deg C, 3 h View Scheme

1: 1.) dibutyltin oxide, 2.) triethylamine / 1.) methanol, 5 h, reflux, 2.) methanol, 1 h, room temperature 2: 88 percent / 40 percent sodium hydrogensulfide aq. / dimethylformamide / 6 h / 60 °C 3: 18 percent / Raney nickel / H2O / 3 h / Heating View Scheme

1: 45 percent / ammonia gas / methanol / 15 h / 100 °C 2: 18 percent / Raney nickel / H2O / 3 h / Heating View Scheme

1: 88 percent / pyridine / 48 h / Ambient temperature 2: phosphoryl chloride, N,N-diethylaniline / 0.08 h / Heating 3: 45 percent / ammonia gas / methanol / 15 h / 100 °C 4: 18 percent / Raney nickel / H2O / 3 h / Heating View Scheme

1: 54 percent / ammonia gas / ethanol / 10 h / 100 °C 2: 92 percent / saturated bromine-water / 1 h / Ambient temperature 3: 1.) dibutyltin oxide, 2.) triethylamine / 1.) methanol, 5 h, reflux, 2.) methanol, 1 h, room temperature 4: 88 percent / 40 percent sodium hydrogensulfide aq. / dimethylformamide / 6 h / 60 °C 5: 18 percent / Raney nickel / H2O / 3 h / Heating View Scheme

1: 88 percent / 40 percent sodium hydrogensulfide aq. / dimethylformamide / 6 h / 60 °C 2: 18 percent / Raney nickel / H2O / 3 h / Heating View Scheme

T=37°C; 7 h; alginate gel-entrapped cells of auxotrophic thymine-dependent strain of E. coli, ammonium acetate buffer pH 5.8;

1: DMAP / acetonitrile / 1 h / Ambient temperature 2: 1.) AIBN, Bu3SnH, 2.) TBAF / 1.) toluene, 80 deg C, overnight, 2.) toluene, THF, 80 deg C, 3 h View Scheme