beta-D-半乳糖基-(1→3)-N-乙酰基-D-半乳糖胺合成路线 （共 5 条）
反应条件
β-D-galactosidase T=37°C; sodium phosphate-citrate buffer (pH 4,3), 0,02percent NaN3;
参考文献
Hedbys, Lars; Johansson, Elisabet; Mosbach, Klaus; Larsson, Per-Olof; Gunnarsson, Alf; Svensson, Sigfrid
Carbohydrate Research, 1989 , vol. 186, p. 217 - 224
反应条件
endo-α-N-acetylgalactosaminidase; manganese (II) chloride T=37°C; pH=6.8; 1 h; aq. bufferEnzymatic reaction;
参考文献
Ju, Tongzhong; Xia, Baoyun; PAryal, Rajindra; Wang, Wenyi; Wang, Yingchun; Ding, Xiaokun; Mi, Rongjuan; He, Miao; Richard D. Cummings
Glycobiology, 2011 , vol. 21, # 3 p. 352 - 362
→
72142-81-5N-[(2R,3R,4R,5R)-3,4,5-三羟基-1-氧代-6-[(2R,3R,4S,5R,6R)-3,4,5-三羟基-6-(羟基甲基)四氢吡喃-2-基]氧基己烷-2-基]乙酰胺+
反应条件
phosphate buffer; β-galactosidase in N,N-dimethyl-formamide T=37°C; pH=6.0; Product distribution; Further Variations:Reagents;
参考文献
Miyasato, Mariko; Ajisaka, Katsumi
Bioscience, Biotechnology and Biochemistry, 2004 , vol. 68, # 10 p. 2086 - 2090
→
72142-81-5N-[(2R,3R,4R,5R)-3,4,5-三羟基-1-氧代-6-[(2R,3R,4S,5R,6R)-3,4,5-三羟基-6-(羟基甲基)四氢吡喃-2-基]氧基己烷-2-基]乙酰胺
反应条件
β-D-galactosidase T=37°C; sodium phosphate-citrate buffer (pH 4,3), 0,02percent NaN3; reactions for var. times; Product distribution;
参考文献
Hedbys, Lars; Johansson, Elisabet; Mosbach, Klaus; Larsson, Per-Olof; Gunnarsson, Alf; Svensson, Sigfrid
Carbohydrate Research, 1989 , vol. 186, p. 217 - 224
反应条件
T=90°C; pH=7.5; aq. phosphate buffer; Kinetics; pH-valueTemperature;
参考文献
Chiku, Kazuhiro; Nishimoto, Mamoru; Kitaoka, Motomitsu
Carbohydrate Research, 2010 , vol. 345, # 13 p. 1901 - 1908
