nuclease-3'-nucleotidase P1; sodium acetate in water T=60°C; 24 h;

1: 5 g / nuclease-3'-nucleotidase P1, CH3COONa, / H2O / 24 h / 60 °C 2: 1.) tri-n-butylamine 2.) H2) / 1.) pyridine, RT, 20 h, 2.) RT, 20 h, 3: 1.) TPS-Cl, / 1.) CHCl3, 2.) pyridine, RT, 5 h, 4: NH3 / ethanol View Scheme