potassium phosphate; wild-type CYP119; dihydrogen peroxide in ethanol; water T=50°C; pH=7.4; Enzymatic reaction; regiospecific reaction;

wildtype P450BM3; NADPH; catalase in phosphate buffer T=37°C; 0.5 h; Title compound not separated from byproducts;

putidaredoxin reducatse; wild type; putidaredoxin in phosphate buffer T=37°C; pH=7.0; Enzyme kineticsProduct distribution; Further Variations:enzymes;

cytochrome P450 BM3 Enzymatic reaction;

potassium hydroxide; sulfuric acid in 1,4-dioxane; ethyl acetate 1) reflux, 30 min; 2) reflux, 30 min;

1: 30 percent / dimethylsulfoxide / 0.5 h / 60 - 70 °C 2: HCl / methanol / 40 h / 0 °C 3: diethyl ether / 20 h / Heating 4: BF3*Et2O / methanol / 2 h 5: 1) Mg, 2) COD*CuCl / 1) THF, 1,2-dibromoethan, reflux, 4 h, 2) THF, 35 deg C, 20 min 6: 1) 0.02N H2SO4; 2) 2N KOH / ethyl acetate; dioxane / 1) reflux, 30 min; 2) reflux, 30 min View Scheme

1: HCl / methanol / 40 h / 0 °C 2: diethyl ether / 20 h / Heating 3: BF3*Et2O / methanol / 2 h 4: 1) Mg, 2) COD*CuCl / 1) THF, 1,2-dibromoethan, reflux, 4 h, 2) THF, 35 deg C, 20 min 5: 1) 0.02N H2SO4; 2) 2N KOH / ethyl acetate; dioxane / 1) reflux, 30 min; 2) reflux, 30 min View Scheme

1: 1) Mg, 2) COD*CuCl / 1) THF, 1,2-dibromoethan, reflux, 4 h, 2) THF, 35 deg C, 20 min 2: 1) 0.02N H2SO4; 2) 2N KOH / ethyl acetate; dioxane / 1) reflux, 30 min; 2) reflux, 30 min View Scheme

flawocytochrome P450 BM3 from Bacillus megaterium W1046A mutant, NADH-dependent fatty acid hydroxylase; Reduced nicotinamide-adenine dinucleotide T=25°C; pH=7; 0.0333333 h; aq. phosphate bufferEnzymatic reaction; regioselective reaction;