胞苷 5'-单磷酸酯 N-乙酰基神经氨酸合成路线 （共 27 条）
反应条件
Tris-HCl buffer; recombinant sialic acid aldolase; magnesium chloride; recombinant Neisseria meningitidis CMP-Neu5Ac synthetase in water T=37°C; pH=8.8;
参考文献
Yu, Hai; Yu, Hui; Karpel, Rebekah; Chen, Xi
Bioorganic and Medicinal Chemistry, 2004 , vol. 12, # 24 p. 6427 - 6435
反应条件
enzymatic synthesis (cytidine 5'-monophosphosialate synthase, inorganic pyrophosphatase);
参考文献
Thiem, Joachim; Stangier, Peter
Liebigs Annalen der Chemie, 1990 , # 11 p. 1101 - 1105
反应条件
Tris-HCl buffer; recombinant sialic acid aldolase; magnesium chloride; recombinant Neisseria meningitidis CMP-Neu5Ac synthetase in water T=37°C; pH=8.8;
参考文献
Yu, Hai; Yu, Hui; Karpel, Rebekah; Chen, Xi
Bioorganic and Medicinal Chemistry, 2004 , vol. 12, # 24 p. 6427 - 6435
反应条件
1.1: 1H-tetrazole / acetonitrile 1.2: tBuOOH / acetonitrile 1.3: DBU / tetrahydrofuran 2.1: NaOMe / methanol 3.1: NaOH View Scheme
参考文献
Chappell, Mark D.; Halcomb, Randall L.
Tetrahedron, 1997 , vol. 53, # 32 p. 11109 - 11120
反应条件
1.1: 1H-tetrazole / acetonitrile 1.2: tBuOOH / acetonitrile 1.3: DBU / tetrahydrofuran 2.1: NaOMe / methanol 3.1: NaOH View Scheme
参考文献
Chappell, Mark D.; Halcomb, Randall L.
Tetrahedron, 1997 , vol. 53, # 32 p. 11109 - 11120
反应条件
inorganic pyrophosphatase (EC 3.6.1.1); Neisseria meningitidis B; recombinant CMP-sialate synthetase; magnesium chloride in water T=20°C; pH=8.5; Enzymatic reaction;
参考文献
Knorst, Marion; Fessner, Wolf-Dieter
Advanced Synthesis and Catalysis, 2001 , vol. 343, # 6-7 p. 698 - 710
反应条件
1: dimethylformamide; acetonitrile 2: NaOMe / methanol 3: NaOH View Scheme
参考文献
Izumi; Shen; Wacowich-Sgarbi; Nakatani; Plettenburg; Wong
Journal of the American Chemical Society, 2001 , vol. 123, # 44 p. 10909 - 10918
反应条件
1.1: 1H-tetrazole / acetonitrile 1.2: tBuOOH / acetonitrile 1.3: DBU / tetrahydrofuran 2.1: NaOMe / methanol 3.1: NaOH View Scheme
参考文献
Izumi; Shen; Wacowich-Sgarbi; Nakatani; Plettenburg; Wong
Journal of the American Chemical Society, 2001 , vol. 123, # 44 p. 10909 - 10918
反应条件
baker's yeast; magnesium chloride in phosphate buffer; xylene T=28°C; pH=8.0; 32 h;
参考文献
Hamamoto, Tomoki; Takeda, So; Noguchi, Toshitada
Bioscience, Biotechnology and Biochemistry, 2005 , vol. 69, # 10 p. 1944 - 1950
反应条件
baker's yeast; D-glucose; magnesium chloride in phosphate buffer; xylene T=28°C; pH=8.0;
参考文献
Hamamoto, Tomoki; Takeda, So; Noguchi, Toshitada
Bioscience, Biotechnology and Biochemistry, 2005 , vol. 69, # 10 p. 1944 - 1950
