sodium hydroxide in ethanol; water T=20°C; 1 h;

1: swine bile With sodium hydroxide 20 h; Heating / reflux; 2: With dihydrogen peroxide in ethyl acetate T=70°C; 3: sodium hydroxide With hydrogenchloride; water more than 3 stages;

ethylenediaminetetraacetic acid; Acinetobacter calcoaceticus lwoffii 7α-hydroxysteroid dehydrogenase; sodium 2-oxopropanoate; nicotinamide adenine dinucleotide; 2-hydroxyethanethiol; lactate dehydrogenase in aq. phosphate buffer T=22°C; pH=8.5; Enzymatic reaction;

acetic acid in water