DMA; β-galactosidase-3-N-terminal 6-histidine tag from Bacillus circulans ATCC 31382 in aq. phosphate buffer T=37 - 100°C; pH=6; Green chemistryEnzymatic reaction; regioselective reaction;

β-GaL-3-NTag from B. circulans ATCC 31382; water; [Aliquat][NTf2] in aq. phosphate buffer T=37°C; pH=6; 3 h; Enzymatic reaction;

β-GaL-3-NTag from B. circulans ATCC 31382; 1-n-butyl-3-methylimidazolium tetrafluoroborate in aq. phosphate buffer T=37°C; pH=6; 3 h; Enzymatic reaction;

galactokinase; D-galactosyl-β1-3-N-acetyl-D-hexosamine phosphorylase from Bifidobacterium infantis; adenosine 5'-triphosphoric acid in water; magnesium chloride T=37°C; pH=6.5; 24 h; aq. bufferEnzymatic reaction;

1: pyridine / 1.) -30 -> 0 deg C, 2.) 6 h, 0 deg C 2: toluene / 48 h / Ambient temperature 3: NaOCH3/CH3OH / 24 h / Ambient temperature 4: H2 / 10percent Pd-C / methanol / 16 h / 4500.4 Torr / Ambient temperature View Scheme

1: 78 percent / HCl, NaBH3CN, 3 Angstroem molecular sieves / diethyl ether; tetrahydrofuran / 0.33 h 2: pyridine / 1.) -30 -> 0 deg C, 2.) 6 h, 0 deg C 3: toluene / 48 h / Ambient temperature 4: NaOCH3/CH3OH / 24 h / Ambient temperature 5: H2 / 10percent Pd-C / methanol / 16 h / 4500.4 Torr / Ambient temperature View Scheme

1: NaOCH3/CH3OH / 24 h / Ambient temperature 2: H2 / 10percent Pd-C / methanol / 16 h / 4500.4 Torr / Ambient temperature View Scheme

1: toluene / 48 h / Ambient temperature 2: NaOCH3/CH3OH / 24 h / Ambient temperature 3: H2 / 10percent Pd-C / methanol / 16 h / 4500.4 Torr / Ambient temperature View Scheme

[3H]-Uridine diphosphate glucose in phosphate buffer T=30°C; pH=7.5; 140 h; Large scale reactionEnzymatic reaction;