2-(n-morpholino)ethanesulfonic acid; VBrPO(AnI) bromoperoxidase; dihydrogen peroxide; sodium bromide in water; tert-butyl alcohol T=20°C; pH=6.2; Overall yield = 51 percent;

1: dihydrogen peroxide; potassium carbonate / dimethyl sulfoxide / 1.5 h / 0 - 20 °C 2: Ascophyllum nodosum vanadate(V)-dependent bromoperoxidase I; dihydrogen peroxide; sodium bromide / water; tert-butyl alcohol / 72 h / 23 °C / pH 6.2 / aq. buffer; Enzymatic reaction View Scheme