thiamine diphosphate; magnesium sulfate in various solvent(s) T=30°C; Rearrangement; pH=7.5; 7 h; Enzymatic reactioninitial compound concentration 1, 10, 50 and 100 mM; reaction was carried out in Tris-HCl buffer;
ammonium sulfate; nicotinamide adenine dinucleotide; pyruvate kinase e; hexokinase in hexane; water 32 h; membran-enclosed enzymatic catalysis;
参考文献
Bednarski, Mark D.; Chenault, H. Keith; Simon, Ethan S.; Whitesides, George M.
Journal of the American Chemical Society, 1987 , vol. 109, # 4 p. 1283 - 1285