1: With hydrogenchloride; sodium hydroxide; ammonium hydroxide in methanol; water; ethyl acetate T=20 - 70°C; 0.166667 h; Heating / reflux; 2: With hydrogenchloride in methanol; water T=20°C; pH=4.8;

sodium hydroxide T=20°C; 0.5 h;

methanol; ammonia; water; esterase SEQ ID NO:4 (encoded by SEQ ID NO:3) in toluene pH=7; Tris bufferEnzymatic reaction; Product distribution / selectivity;

1: N-cyclopropyl-7-[1,2,6,7,8,8a(R)-hexahydro-2(S),6(R)-dimethyl-8(S)-[[2(S)-methylbutanoyl]oxy]-1(S)-naphthyl]-3(R),5(R)-dihydroxyheptanoic acid amine salt With pyrrolidine; n-butyllithium in tetrahydrofuran; hexane T=-45 - -25°C; 2 h; 2: methyl iodide in tetrahydrofuran; hexane T=-30 - -10°C; 1.33333 h; 3: With hydrogenchloride in tetrahydrofuran; hexane; water

enzyme SEQ ID NO:4 (encoded by SEQ ID NO:3) 48 h; Product distribution / selectivity;

T=50°C; pH=7.5; 96 h; Enzymatic reaction; Product distribution / selectivity;

hydrogenchloride in water; ethyl acetate pH=~ 4.0; 0.5 h; Product distribution / selectivity;

enzyme SEQ ID NO:4 (encoded by SEQ ID NO:3) 48 h; Product distribution / selectivity;

enzyme SEQ ID NO:4 (encoded by SEQ ID NO:3) 48 h; Product distribution / selectivity;

Kinetics; Reagent/catalyst;