1: With 2-(n-morpholino)ethanesulfonic acid; copper diacetate; sodium L-ascorbate; potassium iodide; peptidylglycine α-amidating monooxygenase (PAM), rat, EC 1.14.17.3 in water T=37°C; pH=6; 4 h; 2: With trifluoroacetic acid in water; acetonitrile Enzyme kinetics;