1: With chloroformic acid ethyl ester; triethylamine in tetrahydrofuran T=-15°C; 0.166667 h; 2: With ammonium hydroxide; hydrogen in tetrahydrofuran T=0°C; 2 h; 3: With palladium on activated carbon in methanol 3 h;

triethylamine in N,N-dimethyl-formamide

1: Tris buffer pH 8.2 / 168 h / Ambient temperature; L-leucine aminopeptidase 2: NH3 / methanol / 72 h / 0 °C View Scheme

1: Tris buffer pH 8.2 / 168 h / Ambient temperature; L-leucine aminopeptidase 2: NH3 / methanol / 72 h / 0 °C 3: methanol / 1 h / Ambient temperature View Scheme

Tris buffer pH 8.2 168 h; Ambient temperatureL-leucine aminopeptidase;